Epigenetic Control of Tamoxifen-Resistant Breast Cancer
Revised annual summary rept. 15 Dec 2011-14 Dec 2012
MASSACHUSETTS UNIV AMHERST
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The objective is to understand the role that epigenetics, specifically methylation, plays in antiestrogen resistant breast cancer. The goal of this study is to identify genes differentially methylated between acquired tamoxifen resistant cells TMX2-28 and TMX2-11 and their parent strain MCF-7 through the use of the Illumina HumanMethylation450 BeadChip. A panel of genes showing changes in methylation between the tamoxifen resistant cell lines, TMX2-11 and TMX2-28 and the parental line, MCF-7 were selected for further analysis. One gene, ZNF350, which was hypermethylated in both TMX2-11 and TMX2-28 but not in MCF-7, showed significant increase in expression when treated with a demethylase. Additionally, preliminary treatment assays show a decrease in the proliferation rate of TMX2-28 when treated with a demethylase.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research