Accession Number:

ADA581577

Title:

Biomedical Applications of Micro-Raman and Surface-Enhanced Raman Scattering (SERS) Technology

Descriptive Note:

Final rept. 30 Sep 2010-29 Sep 2012

Corporate Author:

HAWAII UNIV HONOLULU

Report Date:

2012-10-01

Pagination or Media Count:

70.0

Abstract:

The purpose of this project was to evaluate Raman-based technologies for early detection of urogenital cancers. Most of the work was directed at improving different components of the surface-enhanced Raman scattering SERS-based immunoassay, analyzing tissue samples with micro-Raman imaging technology, micro-Raman and SERS detection of prostate cancer cells, and SERS detection of exfoliated cells from urine. The most significant findings were as follows. i The nitrocellulose membranes were proven to be an effective media for PSA capture. For SERS-based immunoassays, nitrocellulose offers comparable results to those obtained using gold-coated glass substrates while offering a more cost-effective and time-saving method of detecting minute amounts of PSA ii Micro-Raman imaging technology was found to be effective in chemical mapping of arteries in the tissues of a post mortem individual whose cause of death was a cardiac event iii Both micro-Raman and micro-SERS analysis were able to characterize the biochemistry at the molecular level of three types of prostate cancer cell lines DU145, PC3 and LNCaP and iv Micro-SERS analysis of exfoliated cells from the urine sample of a healthy volunteer showed the potential of the technique as the SERS spectra of individual cells could be measured with 1 s integration time, and various types of cells could be differentiated on the basis of their Raman spectra.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE