Accession Number:
ADA581577
Title:
Biomedical Applications of Micro-Raman and Surface-Enhanced Raman Scattering (SERS) Technology
Descriptive Note:
Final rept. 30 Sep 2010-29 Sep 2012
Corporate Author:
HAWAII UNIV HONOLULU
Personal Author(s):
Report Date:
2012-10-01
Pagination or Media Count:
70.0
Abstract:
The purpose of this project was to evaluate Raman-based technologies for early detection of urogenital cancers. Most of the work was directed at improving different components of the surface-enhanced Raman scattering SERS-based immunoassay, analyzing tissue samples with micro-Raman imaging technology, micro-Raman and SERS detection of prostate cancer cells, and SERS detection of exfoliated cells from urine. The most significant findings were as follows. i The nitrocellulose membranes were proven to be an effective media for PSA capture. For SERS-based immunoassays, nitrocellulose offers comparable results to those obtained using gold-coated glass substrates while offering a more cost-effective and time-saving method of detecting minute amounts of PSA ii Micro-Raman imaging technology was found to be effective in chemical mapping of arteries in the tissues of a post mortem individual whose cause of death was a cardiac event iii Both micro-Raman and micro-SERS analysis were able to characterize the biochemistry at the molecular level of three types of prostate cancer cell lines DU145, PC3 and LNCaP and iv Micro-SERS analysis of exfoliated cells from the urine sample of a healthy volunteer showed the potential of the technique as the SERS spectra of individual cells could be measured with 1 s integration time, and various types of cells could be differentiated on the basis of their Raman spectra.
Descriptors:
Subject Categories:
- Anatomy and Physiology
- Medicine and Medical Research