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Biomedical Applications of Micro-Raman and Surface-Enhanced Raman Scattering (SERS) Technology

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Final rept. 30 Sep 2010-29 Sep 2012

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The purpose of this project was to evaluate Raman-based technologies for early detection of urogenital cancers. Most of the work was directed at improving different components of the surface-enhanced Raman scattering SERS-based immunoassay, analyzing tissue samples with micro-Raman imaging technology, micro-Raman and SERS detection of prostate cancer cells, and SERS detection of exfoliated cells from urine. The most significant findings were as follows. i The nitrocellulose membranes were proven to be an effective media for PSA capture. For SERS-based immunoassays, nitrocellulose offers comparable results to those obtained using gold-coated glass substrates while offering a more cost-effective and time-saving method of detecting minute amounts of PSA ii Micro-Raman imaging technology was found to be effective in chemical mapping of arteries in the tissues of a post mortem individual whose cause of death was a cardiac event iii Both micro-Raman and micro-SERS analysis were able to characterize the biochemistry at the molecular level of three types of prostate cancer cell lines DU145, PC3 and LNCaP and iv Micro-SERS analysis of exfoliated cells from the urine sample of a healthy volunteer showed the potential of the technique as the SERS spectra of individual cells could be measured with 1 s integration time, and various types of cells could be differentiated on the basis of their Raman spectra.

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  • Anatomy and Physiology
  • Medicine and Medical Research

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