Using 3-D Super-Resolution Microscopy to Probe Breast Cancer Stem Cells and Their Microenvironment
Annual rept. 15 February 2012 - 14 February 2013
CALIFORNIA UNIV BERKELEY
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We report the achievements of the second year of our project. These achievements include development of a high-throughput platform consisting of an array of microposts of different size to investigate the effect of stiffness range on breast-cancer phenotype given that tumors are heterogeneous in terms of stiffness. We are currently focusing on the MCF-7 a-CD271 phenotype since it has been recently reported to be a candidate breast CaSCs. Through immunostaining, we have identified that phenotype with our MCF-7 cell line. We intend to incorporate microfluidic channels that will deliver specific chemical cues to cells growing on the micropost arrays to best mimic the tumor microenvironment. We will also use our super-resolution microscope developed in Year 1 to image the cells growing to determine spatial pattern changes of surface receptors.
- Medicine and Medical Research