Genetics of Persister Formation in Pseudomonas aeruginosa
Final rept. 1 Jun 2009-31 May 2012
NORTHEASTERN UNIV BOSTON MA OFFICE OF SPONSORED PROJECTS
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Our original proposal was aimed at understanding the mechanism of how Pseudomonas aerugionsa forms drug tolerant persister cells. This is of critical importance because persisters can lead to antimicrobial therapy failure. Specific Aim 1 Transcriptomic analysis of stationary phase persisters. We successfully developed a method to isolate P. aerugionsa persisters and obtained total RNA from these cells. Attempts to obtain a transcriptome with this RNA failed due to the limitations of our samples. However, the methods developed from this work allowed us to obtain the first persister proteome. This achievement is an important technical and scientific advance. The results have given us candidate persister genes that we are now working to validate. Additionally, these results will provide a guide for other groups working on proteomics approaches where material is limited. Specific Aim 2 Genomic Identification of High Persister hip Mutants. We completed this aim and obtained genome sequences of hip mutants from clinical isolates.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research