Infection of the Gulf Coast Tick, Amblyomma Maculatum (Acari: Ixodidae), with Rickettsia Parkeri: First Report from the State of Delaware
NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD RICKETTSIAL DISEASES DEPT
Pagination or Media Count:
The molecular detection of Rickettsia parkeri in a Gulf Coast tick, Amblyomma maculatum, collected in Delaware represents the first evidence of the human pathogen R. parkeri associated with A. maculatum in the state. A total of five adult 2 male and 3 female Gulf Coast ticks were collected from tick drags conducted during a two-day sampling event 21 22 May 2012 at Bombay Hook National Wildlife Refuge, near Smyrna, Delaware. All specimens were tested for the presence of Rickettsia with a genus-specific quantitative real-time polymerase chain reaction qPCR assay one of the female specimens tested positive. This specimen was then assessed for the presence of Rickettsia parkeri and Candidatus Rickettsia andeanae by two species-specific qPCR assays. The presence of R. parkeri DNA was detected, whereas Candidatus R. andeanae DNA was not.