Massively Parallel Rogue Cell Detection Using Serial Time-Encoded Amplified Microscopy of Inertially Ordered Cells in High-Throughput Flow
Final rept. 1 Aug 2010-31 Jul 2012
CALIFORNIA UNIV LOS ANGELES
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We developed a high-throughput imaging cytometry system for high accuracy identification of rare circulating breast cancer cells in blood. Key accomplishments include 1. Construction of a serial time encoded amplified microscopic camera, Nomarski system, and laser scanner for blur flee imaging of breast cancer cells and blood cells in flow with and without microparticle labels to EpCAM on the cell surfaces. 2. Development of microfluidic devices compatible with the optical system in rigid optically transparent materials. 3. Development of a real-time field programmable gate array image analyzer for high-throughput image-based screening, and 4. Inline sample preparation microfluidic system for inline microparticle labeling of breast cancer cells without lossy centrifugation steps. The work has yielded high accuracy 75 identification of spiked breast cancer cells from blood with a false positive rate of 1 MCF7 breast cancer cell in 1,000,000 white blood cells, a few orders of magnitude better accuracy than flow cytometry scatter and fluorescence measurements alone.
- Medicine and Medical Research