Exploration into the Genetics of Food Allergy
Annual rept. 30 Sep 2011-29 Sep 2012
CHILDREN'S HOSPITAL OF PHILADELPHIA PA
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Food Allergies occur in 2-6 of children in the United States. The reactions can be mild to severe and life threatening and potential fatal. No study has been able to identify genetic causes of IgE mediated food allergies. In our previous work, we identified polymorphism in thymic stromal lymphopoietitin region as risk factor for Eosinophilic Esophagitis, a non-IgE mediated disease. This work has examined the genetics of food allergy looking at both IgE and non-IgE mediated food allergy using Genome Wide Analysis GWAS. We have collected samples from patients at The Children s Hospital of Philadelphia, University of Colorado Children s Hospital, Riley s Children Hospital-San Diego, CA Packard Children s Hospital-Stanford, CA. The samples and analysis was split into IgE mediated allergies and Eosinophilic Esophagitis. For patients with Eosinophilic Esophagitis, we have collected over 700 samples. Our preliminary results confirmed the importance of TSLP region as a risk factor for Eosinophilic Esophagitis with p value of 10-12. Two additional regions have been identified and now being confirmed using a replicative cohort. For the patients with IgE mediated allergies, we have collected families and done initial GWAS analysis on previous collected samples of 800 patients with no identified regions. Therefore, we have done exon sequencing of 10 family cohorts with normal controls. Multiple areas of interest have been identified and awaiting confirmation via alternative methods. The projects also examined genotype-phenotype relationships. Using novel component analysis, we are able to identify serological markers that identified patients at risk for a severe reaction.
- Anatomy and Physiology
- Medicine and Medical Research
- Food, Food Service and Nutrition