Mass Scale Biosensor Threat Dianostic for In-Theater Defense Utilization
Annual rept. 20 Sep 2010-19 Sep 2011
FLORIDA INTERNATIONAL UNIV MIAMI
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The goals of this project are to develop two biosensors capable of rapidly assessing exposure to unknown toxicants and to validate these biosensors by gold-standard cytogenetic assays. We have made significant progress in all three areas of the project. IRB documentation has been completed and 9 subjects recruited for blood draws. H2O2 has been demonstrated to increase the frequency and severity of chromosome aberrations with increasing concentrations. Two biosensors have been developed. For the EIST biomarker we demonstrated the feasibility of detecting the 8-OHdG biomarker using a disposable paper-based strip and screen printed electrodes, which provides the fundamental underpinning for integration of electrodes to paper strips for quantitative measurements of 8-OHdG. For the gold nanoparticle whole-cell based biosensor, we have completed dose and time kinetics studies to quantify the expressed stress proteins Hsp70 and Rad54, in response to environmental stress H2O2 and UV using ELISA. We have successfully measured standard forms of Caspase-3, RAD54 and Hsp70 using the 3-D Silver colloidal nanosphere as SERS substrate and demonstrated that Caspase-3 can be measured to 1 ngml below the level of detection by commercial ELISA kits-4.8 ngml. During the next year we will assess the accuracy and reliability of these 2 biosensors.
- Chemical, Biological and Radiological Warfare
- Miscellaneous Detection and Detectors