Designed Proteins as Optimized Oxygen Carriers for Artificial Blood
Annual rept. 10 Jan 2012-9 Jan 2013
CITY COLL NEW YORK
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This project concerns the transformation of a de novo designed oxygen transport protein into an effective blood substitute. Second year accomplishments include A The creation and verification of a theoretical model for oxyferrous state lifetimes in oxygen transport proteins. B We have modified global protein dynamics to double of oxyferrous state lifetime. C We have used surface side chain charges to increase the reduction potential of the bound cofactor and triple the oxyferrous state lifetime. D We have incorporated an optimized cofactor binding site and shown that the decrease in protein core dynamics increases the lifetime four-fold. E We have made significant progress on the determination of the three-dimensional structure of one of the best performing variants. After a limited amount of additional optimization, this protein is ready for incorporation into the crosslinked particles that are the goal of year three.
- Anatomy and Physiology
- Medicine and Medical Research