Accession Number:

ADA574241

Title:

Designed Proteins as Optimized Oxygen Carriers for Artificial Blood

Descriptive Note:

Annual rept. 10 Jan 2012-9 Jan 2013

Corporate Author:

CITY COLL NEW YORK

Personal Author(s):

Report Date:

2013-02-01

Pagination or Media Count:

13.0

Abstract:

This project concerns the transformation of a de novo designed oxygen transport protein into an effective blood substitute. Second year accomplishments include A The creation and verification of a theoretical model for oxyferrous state lifetimes in oxygen transport proteins. B We have modified global protein dynamics to double of oxyferrous state lifetime. C We have used surface side chain charges to increase the reduction potential of the bound cofactor and triple the oxyferrous state lifetime. D We have incorporated an optimized cofactor binding site and shown that the decrease in protein core dynamics increases the lifetime four-fold. E We have made significant progress on the determination of the three-dimensional structure of one of the best performing variants. After a limited amount of additional optimization, this protein is ready for incorporation into the crosslinked particles that are the goal of year three.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE