Pediatric Glioblastoma Therapies Based on Patient-Derived Stem Cell Resources
Annual summary rept. 1 Sep 2011-31 Aug 2012
FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA
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Gliomas comprise 60 of cases among pediatric brain tumors. To date, there are few resources available to study and manipulate pediatric brain tumor cells, to evaluate whether pediatric tumor will have fundamental different responses to the new therapeutic regimes. Since glioma stem cell lines have been successfully isolated from adults, in this proposal we aim to isolate GSC population from pediatric patients. Through a collaboration with Dr. Xiao-Nan Li at Texas Children s Hospital, in the past year, Dr. Paddison s group has successfully derived and cultured eight patient-derived pediatric glioma stem cell lines and has begun molecular and phenotypic characterization of these lines. This characterization has included analysis of gene expression and patient-specific gene mutations, and also proof-of-concept shRNA screens. As a direct result of these efforts, we have begun to observe similarities and differences among pediatric glioma isolates among genes and networks required for proliferation and survival the key objective of this grant.
- Medicine and Medical Research