Hydrogen-bonded LbL Shells for Living Cell Surface Engineering
AIR FORCE RESEARCH LAB WRIGHT-PATTERSON AFB OH HUMAN EFFECTIVENESS DIRECTORATE
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We report on the design of cytocompatible synthetic shells from highly permeable, hydrogen-bonded multilayers for cell surface engineering with preservation of long-term cell functioning. In contrast to traditional polyelectrolyte layer-by-layer LbL systems, shells suggested here are based on hydrogen bonding allowing gentle cell encapsulation using non-toxic, non-ionic and biocompatible components such as polyN-vinylpyrrolidone PVPON and tannic acid TA which were earlier exploited on abiotic surfaces but never assembled on cell surfaces. Here, we demonstrate that these LbL shells with higher diffusion facilitate outstanding cell survivability reaching 79 in contrast to only 20 viability level achieved with ionically paired coatings. We suggest that the drastic increase in cell viability and preservation of cell functioning after coating with synthetic shell stems from the minimal exposure of the cells to toxic polycations and high shell permeability.
- Polymer Chemistry