Accession Number:

ADA568972

Title:

Selective Gene Regulation by Androgen Receptor in Prostate Cancer

Descriptive Note:

Annual summary 30 Sep 2011-29 Sep 2012

Corporate Author:

MICHIGAN UNIV ANN ARBOR

Personal Author(s):

Report Date:

2012-10-01

Pagination or Media Count:

12.0

Abstract:

Androgen binds to the androgen receptor AR and is required for prostate cancer initiation and progression. Although androgen ablation is initially an effective prostate cancer therapy, patients eventually develop resistance, but disease remains driven by AR. There is a need to identify AR gene programs that promote proliferation versus differentiation to design better treatments. Using mutant ARs as models of aberrant AR activity, I generated cell lines harboring wild-type and mutant ARs and analyzed proliferation, anchorage-independent cell growth and alterations in AR target genes. I also generated a multiplexed promoter assay for high-throughput screening HTS to identify compounds that selectively regulate AR. After assay optimization, the primary HTS was performed with 2,5000 compounds. A dose response assay identified several compounds that selectively regulate AR and will be further studied in cell-based assays.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE