SEPT9_v1 Functions in Breast Cancer Cell Division
Revised annual summary rept. 1 Jan 2009-31 Dec 2011
LELAND STANFORD JUNIOR UNIV CA
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Septins comprise a large, conserved family of GTPases that fassemble into apolar filaments, bundles and rings. Septins play important roles in mitosis, cell migration, and cell morphogenesis by forming scaffolds and diffusion barriers. Defects in septin expression, organization and function are implicated in many human diseases including cancer and neuropathies. During this period of funding 1. We tested the effect of a plant cytokinin, Forchlorfenuron FCF, on mammalian septins, and showed that FCF effects septin assembly, and induced mitotic and cell migration defects 2. We showed that in mammalian epithelial cells that vesicles containing apical or basolateral proteins exit the Golgi complex along SEPT2polyGlu microtubule tracks, and that these tracks are required for the proper morphogenesis of polarized epithelia 3. We showed that SEPT2 is part of a diffusion barrier at the base of the primary cilium, which transduces extracellular signals through signaling receptors localized in the ciliary membrane, and is essential for retaining receptor-signaling pathways in the primary cilium. In summary, the results of this funding have provided significant advances in defining new functions for septins that provide novel insights into cellular functions and defects that could occur in human diseases.
- Medicine and Medical Research