Global Characterization of Protein-Altering Mutations in Prostate Cancer
Annual rept. 15 Jul 2011-14 Jul 2012
FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA
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The identification of recurrent, protein-altering genetic alterations is frequently the means by which a given gene is initially implicated in tumor biology. Our specific aims are 1 Carry out the genome-wide identification of nonsynonymous mutations in prostate metastases 2 Evaluate the mutational histories of individual mutations within the progression of the cancer in which it was observed, and assess prevalence 3 Perform integrative analyses of somatic mutation with gene expression and copy number change data collected on the same samples. During this funding period we have i completed the identification and quality assessment of the prostate cancer tissuessamples that will be used for the project ii completed DNA isolation for 300 samplescases of prostate cancer iii Transfered the 1 st and 2 nd groups of DNAs to the Shendure lab for Exome analysis iv completed exome sequencing on 50 tumor metastasis and completed the analysis of 27 of these exomes v completed CGH assays on 200 prostate cancers vi initiated the integrated analyses of gene expression, copy number and mutation in prostate cancer using network approaches.
- Medicine and Medical Research