Accession Number:

ADA568168

Title:

Origins of DNA Replication and Amplification in the Breast Cancer Genome

Descriptive Note:

Final rept. 1 Sep 2010-31 Aug 2012

Corporate Author:

BROWN UNIV PROVIDENCE RI

Report Date:

2012-09-01

Pagination or Media Count:

43.0

Abstract:

The major goal of this IDEA Expansion Breast Cancer Research grant is to test whether a correlation existsbetween sites of DNA amplification and estrogen receptor ER binding in the breast cancer genome. Correlations would support our hypothesis that ER adjacent to replication origins may interact with the replication machinery to drive DNA amplification, a hallmark of many cancers. Our Specific Aims are 1 Map replication origins in the MCF-7 breast cancer genome by genomic sequencing 2 Compare the replication origin maps between breast cancer ER, ER- and normal breast cells 3 Correlate the origin map data with sites of DNA amplification and estrogen receptor binding 4 Pilot runs to map replication origins in ER human breast cancer tissue and sites of DNA amplification

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE