Preclinical Studies of Induced Pluripotent Stem Cell-Derived Astrocyte Transplantation in ALS
Annual rept. 30 Sep 2011-29 Sep 2012
JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
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We have made substantial progress in obtaining skin biopsies with subsequent fibroblast cultures of a number of sporadic ALS, familial ALS, and control subjects in the last year. We have created numerous iPS cell lines from these patient samples and have characterized their long-term differentiation into astroglia. We now have data from the in vivo transplantation of these astroglial progenitors into rat spinal cords. We have characterized several control and 1 ALS cell line with regard to their survival, differentiation, migration, and phenotypic effects on the host animals. Our data demonstrate that there are differences amongst the cell lines with regard to these properties but we have not appreciated obvious differences with regard to ALS cells when compared with control astroglial cells. Finally, we have demonstrated, in preparation for our therapeutic approaches, that these glial progenitor cells continue to mature into astrocytes following transplantation.
- Anatomy and Physiology
- Medicine and Medical Research