Accession Number:

ADA568118

Title:

Studies of the Tumor Microenvironment in Pathogenesis of Neuroblastoma

Descriptive Note:

Annual rept. 1 Jul 2011-30 Jun 2012

Corporate Author:

CHILDREN'S HOSPITAL LOS ANGELES CA

Personal Author(s):

Report Date:

2012-07-01

Pagination or Media Count:

8.0

Abstract:

The NBL-Tag neuroblastoma mice were crossed with B-cell deficient mice in order to determine the effect of B-cell activity on tumor growth characteristics. Homozygous deficient B-Cell mice and heterozygous NBL-Tag mice were established. The NBL-TagB-cell deficient mice lacked B-cells as expected using flow cytometry analyses and immunohistochemistry. However, the lack of B-cells did not alter the growth patterns of NBL-Tag tumor formation as imaged by MRI. Studies using anti-B cell therapy were also conducted. The efficacy of the anti-mouse B-cell antibody was tested in wildtype C57B6 mice tissues blood, spleen, and peritoneal cavity lymphocytes and demonstrated superiority of the antibody to eliminate B-cells when given in the peritoneum. NBL-Tag mice given this antibody starting at 4 weeks of age did not show any difference in their growth characteristics. The anti-B cell antibody combination with chemotherapy in established tumors showed superiority compared with anti-Ragweed antibody control. Further studies are underway to elucidate the effect of macrophages on tumor formation and growth.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE