Studies of the Tumor Microenvironment in Pathogenesis of Neuroblastoma
Annual rept. 1 Jul 2011-30 Jun 2012
CHILDREN'S HOSPITAL LOS ANGELES CA
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The NBL-Tag neuroblastoma mice were crossed with B-cell deficient mice in order to determine the effect of B-cell activity on tumor growth characteristics. Homozygous deficient B-Cell mice and heterozygous NBL-Tag mice were established. The NBL-TagB-cell deficient mice lacked B-cells as expected using flow cytometry analyses and immunohistochemistry. However, the lack of B-cells did not alter the growth patterns of NBL-Tag tumor formation as imaged by MRI. Studies using anti-B cell therapy were also conducted. The efficacy of the anti-mouse B-cell antibody was tested in wildtype C57B6 mice tissues blood, spleen, and peritoneal cavity lymphocytes and demonstrated superiority of the antibody to eliminate B-cells when given in the peritoneum. NBL-Tag mice given this antibody starting at 4 weeks of age did not show any difference in their growth characteristics. The anti-B cell antibody combination with chemotherapy in established tumors showed superiority compared with anti-Ragweed antibody control. Further studies are underway to elucidate the effect of macrophages on tumor formation and growth.
- Anatomy and Physiology
- Medicine and Medical Research