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Accession Number:
ADA566934
Title:
Microfluidic Flow Retardation Device for Tagless Cancer Cell Analysis for Metastatic Potential
Descriptive Note:
Final rept. 1 Jul 2010-30 Jun 2012
Corporate Author:
GENOME DATA SYSTEMS INC TRENTON NJ
Report Date:
2012-07-01
Pagination or Media Count:
15.0
Abstract:
We developed a microfluidic device to detect surface protein expression in individual cancer cells in small cell populations without prior labeling. We used LM2 cells kind gift of Juan Massague, Memorial Sloan-Kettering Cancer Center, NY, derived from murine lung metastases of MDA-MB-231 cells with a unique lung metastatic potential. We selected LM2 cells for 100 sustained expression of IL-13R 2 and cells without expression. We coated the channel with an antibody to IL-13R 2 or with BSA and directed cells over the patch by a microsyringe pump at flow rates of nanoliters per second. The transient interactions with surface ligands resulted in cell flow retardation in cells expressing the cognate surface protein over the antibody-coated patches and rapid, unimpeded flow on BSA or in the absence of the antigen with 100 sensitivity and 100 specificity. We detected and tracked cells with microscope objectives and computer tracking programs. We were able to multiplex 6 surface proteins creating a barcode for their expression in each individual cell in a population. We characterized cells in mixed tumors using needle aspirates. All objectives were achieved.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
