Accession Number:

ADA566873

Title:

Massively Parallel Rogue Cell Detection Using Serial Time-Encoded Amplified Microscopy of Inertially Ordered Cells in High Throughput Flow

Descriptive Note:

Annual rept. 1 Aug 2010-31 Jul 2011

Corporate Author:

CALIFORNIA UNIV LOS ANGELES

Personal Author(s):

• Jalali, Bahram
• Di Carlo, Dino

Report Date:

2011-08-01

Pagination or Media Count:

13.0

Abstract:

The identification of cancer cells CTCs in patient blood has shown to be useful for predicting patient prognosis after primary treatment, and small amounts of CTCs have also been observed in early stage breast cancers. Current gold standard techniques to isolate CTCs based on immunomagnetic capture, however, have low throughput leading to high statistical uncertainty in early stage disease when CTC numbers are very low 1 cellmL. Revolutionary new techniques are required to screen statistically significant larger volumes of blood for CTCs in a cost effective manner. This will critically enable applications in early detection of occult breast cancer not detectable by standard mammography and ultrasonography and dramatically reduce breast cancer related deaths. During this performance period, we successful completed the development of the STEAM flow analyzer and demonstrated real-time detection of rare MCF7 cells in lysed blood with the analyzer with sensitivity of 75 and a false positive rate of 1 in 1,000,000 white blood cells.

Descriptors:

• *BREAST CANCER
• BLOOD
• BLOOD CELLS
• IDENTIFICATION
• LEUKOCYTES
• MICROSCOPY

Subject Categories:

• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE