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Multifunctional Virus-Nanoshell Assembly for Targeted Hyperthermia and Viral Gene Therapy for Breast Cancer
Final rept. 1 Jun 2011-31 May 2012
RICE UNIV HOUSTON TX
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We aimed to develop a virus-Au NS assembly to specifically target breast cancer cells, and to use localized heat of NIR radiation of Au NS to destroy breast cancer cells in synergy with gene therapy. We proposed to develop virus-nanoshell assemblies by attaching adeno-associated virus AAV to gold nanoshells Au NS through chemical bonds. We have successfully completed majority of tasks 1 and 2 of our Statement of Work. Specifically, we have designed and synthesized a linker molecule with dual functionalities to be able to covalently attach AAV to Au NS surface. Au NS was successfully functionalized with the linker molecules. AAV capsid surface was modified with a benzaldehyde functional group. Using higher titer AAV8, we were able to confirm and quantify the successful conjugation. Recombinant AAV vectors encoding a gene under regulation of a heat-responsive promoter were generated. By combining breast cancer targeting, gene therapy, and hyperthermia, the virus-NS assemblies have the potential to greatly reduce the recurrence of cancer, reduce side effects, increase patient survival rates, and improve the quality of life of breast cancer patients.
APPROVED FOR PUBLIC RELEASE