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Early Prediction of Lupus Nephritis Using Advanced Proteomics
Final rept. 1 Jun 2007-31 May 2012
CHILDREN'S HOSPITAL MEDICAL CENTER CINCINNATI OH
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The purpose of this project is to identify initial biomarker patterns in SLE nephritis using screening proteomic profiling. Utility of one of the biomarkers NGAL in predicting worsening of global and renal SLE disease activity has been validated. We have identified novel urinary biomarkers that distinguish between class IV and class V lupus nephritis, including albumin fragments and -1 acid glycoproteinAGP by 2D gel electrophoresis, transferrin Tf, ceruloplasmin Cp, lipocalin-type prostaglandin-D synthetase L-PGDS, and a1-acid glycoprotein AGP by SELDI-TOF-MS, citrate, taurine and hippurate by NMR spectroscopy-based metabolomic profiling, and apolipoprotein D, lipocalin-like prostaglandin D synthetase, hemopexin, ceruloplasmin, -1 acid glycoprotein and orosomucoid by LCMSMS profiling. We have validated whether these biomarkers are differentially expressed in patients with kidney biopsy-proven lupus nephritis types IV and V. Overall, these studies have identified non-invasive biomarkers that identify lupus nephritis sub-classes, and predict the clinical course of the disease. The significance of such biomarkers is that they will provide novel non-invasive tools to identify patients with lupus nephritis, to risk-stratify the subjects for therapies, and to follow the efficacy of therapies.
APPROVED FOR PUBLIC RELEASE