Accession Number:

ADA566171

Title:

Reparative Medicine: Production of Erythrocytes & Platelets from Human Embryonic Stem Cells

Descriptive Note:

Final technical rept. 1 Jun-31 Dec 2011

Corporate Author:

UNIVERSITY OF SOUTH FLORIDA TAMPA

Personal Author(s):

Report Date:

2012-10-15

Pagination or Media Count:

6.0

Abstract:

This project was focused on production of megakaryocytes and platelets from human pluripotent stem cells - originally human embryonic stem cells hESC, but subsequently, as the technology became available, from human induced pluripotent stem cells hiPSC. We developed a serum-free system, and also created our own cell-free extracellular matrix extracts instead of live feeder cells to support hESC and hiPSC growth and maintenance, followed by feeder cell-free cultures to create embryoid bodies, then mesenchymal cells, which then were induced to differentiate into functional megakaryocytes and platelets.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE