Accession Number:

ADA566123

Title:

Human Neural Cell-Based Biosensor

Descriptive Note:

Quarterly technical rept. 1 Jul-30 Sep 2012

Corporate Author:

ARUNA BIOMEDICAL INC ATHENS GA

Personal Author(s):

• Stice, Steven L.
• Chilton, Jamie

Report Date:

2012-10-31

Pagination or Media Count:

5.0

Abstract:

The purpose of this project is to develop in vitro, cell based biosensors for environmental toxins. By using ArunA s neural cell lines derived from both human embryonic stem cells hESCs and human induced pluripotent stem cells hiPSCs, our goal is to provide a human neural cell based biosensor that is a more biologically relevant model of human physiology. This report describes the progress made in these major areas 1 directed differentiation of hESC- and hiPSC-derived neural progenitor cells into dopaminergic neurons 2 directed differentiation of hESC- and hiPSC-derived neural progenitor cells into astrocytes 3 cell culture medium development for the maintenance and differentiation of ArunA s neural cell lines as sensor elements for neurotoxicity and 4 label-free, adhesive signature-based microfluidic cell separation.

Descriptors:

• *CELLS(BIOLOGY)
• *NERVE CELLS
• *STEM CELLS
• *TOXINS AND ANTITOXINS
• BIOLOGICAL DETECTION
• EMBRYOS
• IN VITRO ANALYSIS
• MOTOR NEURONS
• NERVOUS SYSTEM
• PRECURSORS

Subject Categories:

• Medicine and Medical Research
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE