Accession Number:

ADA566042

Title:

Rapid Parallel Screening for Strain Optimization

Descriptive Note:

Quarterly status rept. 17 May-16 Aug 2012

Corporate Author:

J CRAIG VENTER INST ROCKVILLE MD

Personal Author(s):

• Merryman, Chuck

Report Date:

2012-08-16

Pagination or Media Count:

5.0

Abstract:

Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. About half of the desired chemicals have already been screened. Enrichment cultures were used to isolate microbes that utilize these compounds as their sole carbon and energy source. The results are better than predicted and easily surpass the first milestone of approximately 50 microbes growing on at least 10 different biochemicals. The current results substantiate a minor conjecture of the proposal and indicate that any chemical made by one organism is likely to be used as food by another. In preparation for sequencing and biosensor elucidation, we recommend that growth screens continue as forecasted in the proposal.

Descriptors:

• *BIOCHEMISTRY
• *MICROORGANISMS
• BIOLOGICAL DETECTION
• FERMENTATION
• OPTIMIZATION

Subject Categories:

• Biochemistry
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE