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Novel Function of NIBP in Breast Cancer
Annual rept. 1 May 2011-30 Apr 2012
TEMPLE UNIV PHILADELPHIA PA
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The purpose is to identify the potential role of a novel protein NIBP in regulating the tumorigenesis of breast cancer. The scope covers human breast tissue, cancer cell lines and conditional knockout mice. During the first year of the funding period, we have profiled the expression pattern of NIBP at both mRNA and protein levels in various types and stages of breast cancer by qPCR and TMA immunostaining, showing strong correlation of NIBP overexpression with the progression, metastasis and prognosis of breast cancer. We have optimized ELISA for determination of NIBP in patient serum. We determined the important role of NIBP in breast cancer cell lines and initiated the xenograft animal model. Using various deletion and site-directed mutants of NIBP, NIK and IKK2, we identified the interacting domains among them and characterized their structural-functional correlations. We cloned and characterized lentiviral constitutive and Tet-On inducible NIBP expression vectors in HEK293T cells but failed to obtain high efficiency of lentivirus packaging that limited the application to breast cancer cell line and xenograft studies. We obtained two lines of NIBP floxed mouse but failed to induce efficient knockout of NIBP in cell-specific manner. A third line of NIBP floxed mouse is being developed.
APPROVED FOR PUBLIC RELEASE