Genomic Analysis of Complex Microbial Communities in Wounds
Final addendum rept. 2 Jun 2010-1 Jun 2012
TRANSLATIONAL GENOMICS RESEARCH INST PHOENIX AZ
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The work that we conducted was aimed at moving wound research beyond the limitations of culture-based microbial analyses to enable comprehensive characterization of wound colonization and to fully evaluate its impact on healing. We successfully developed a novel, culture-independent approach for characterizing wound microbiota we succeeded in applying statistical tools to begin characterizing the impact of antibiotic therapies and diabetes on wound microbiota we used our novel strategies to define macro-scale spatial variation in chronic wound microbiota we established research partnerships with MIHS to conduct prospective cohort studies on burn and chronic wounds we enrolled a total of 71 patients into a prospective study of burn wound colonization and collected over 700 wound samples from these patients we developed new protocols to isolate bacterial DNA from burn wounds with minimal exogenous DNA contamination we developed a novel bioinformatics pipeline for identifying robust real time PCR targets for detection and quantification of clinical pathogens and developed a comprehensive panel of TaqMan assays n 108 for detection of extraintestinal virulence factors in E. coli.
- Medicine and Medical Research