Tissue and Circulating Expression of IL-1 Family Members Following Heat Stroke
ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA THERMAL AND MOUNTAIN MEDICINE DIVISION
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Interleukin-1 IL-1 is thought to have a significant role in the pathophysiology of heat stroke HS, although little is known regarding the actions or expression patterns of the IL-1 family. This study tested the hypotheses that following HS IL-1 family gene expression is dynamic, while loss of IL-1 signaling enhances recovery. IL-1 family expression was determined in plasma, spleen, and liver from C57BL6J mice n 24 control, n 20 HS at maximum core temperature Tc,Max, hypothermia, and 24 h post-HS 24 h. Soluble IL-1 receptor subtype I sIL-1RI protein expression peaked at 24 h 14,659.01 2,016.28 pgml, P 0.05, while sIL-1RII peaked at hypothermia 19,099.30 1,177.07 pgml. IL-1 alpha gene expression in the spleen ninefold and liver fourfold along with IL-1RI threefold spleen and fivefold liver were maximal at hypothermia. Spleen IL-1 alpha gene expression peaked at Tc,Max fourfold but at hypothermia fourfold in liver. Gene expression of the IL-1 family member IL-18 peaked 2.5-fold at Tc,Max but was similar at all other time points.
- Stress Physiology