Accession Number:

ADA556898

Title:

A New Model for the Estimation of Cell Proliferation Dynamics Using CFSE Data

Descriptive Note:

Technical rept.

Corporate Author:

NORTH CAROLINA STATE UNIV AT RALEIGH CENTER FOR RESEARCH IN SCIENTIFIC COMPUTATION

Personal Author(s):

• Banks, H. T.
• Sutton, Karyn L.
• Thompson, W. C.
• Bocharov, Gennady
• Doumic, Marie
• Schenkel, Tim
• Argilaguet, Jordi
• Giest, Sandra
• Peligero, Cristina
• Meyerhans, Andreas

Report Date:

2011-08-20

Pagination or Media Count:

34.0

Abstract:

CFSE analysis of a proliferating cell population is a popular tool for the study of cell division and division-linked changes in cell behavior. Recently, a partial differential equation PDE model to describe lymphocyte dynamics in a CFSE proliferation assay was proposed. We present a significant revision of this model which improves the physiological understanding of several parameters. Namely, the parameter used previously as a heuristic explanation for the dilution of CFSE dye by cell division is replaced with a more physical component, cellular autofluorescence. The rate at which label decays is also quantified using a Gompertz decay process. We then demonstrate a revised method of fitting the model to the commonly used histogram representation of the data. It is shown that these improvements result in a model with a strong physiological basis which is fully capable of replicating the behavior observed in the data.

Descriptors:

• *CELL DIVISION
• FLUORESCENT DYES
• INVERSE PROBLEMS
• LYMPHOCYTES
• MATHEMATICAL MODELS
• PARTIAL DIFFERENTIAL EQUATIONS

Subject Categories:

• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE