Regulation of Breast Cancer Stem Cell Trafficking and Metastasis by Brain Originated Signals
Revised final rept. 1 Sep 2009-31 Aug 2010
BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA
Pagination or Media Count:
The hypothesis of this study was that adrenergic signaling contributes to reducing the synthesis of CXCL12 by stromal cells, thereby promoting the release of breast tumor-initiating cells in the circulation and that the sympathetic nervous system controls the retention of breast cancer stem cells to their niche through the regulated expression of CXCL12. To address this hypothesis, we examined the role of CXCR4 CXCL12 in breast cancer stem cell mobilization of GFPMDA- MB-231 cells in the presence or absence of AMD3100. AMD3100 is a specific antagonist for CXCR4. The routine identification of single tumor cells in the bone marrow using HE stain, proved difficult. GFP positive cells could be detected in the peripheral blood only if these cells are injected i.v. at 106 cells at least. Therefore, the trafficking of cancer stem cells from the mammary glad to the circulation is a rare event that can over a long period of time.
- Medicine and Medical Research