Regulation of Mammary Tumor Formation and Lipid Biosynthesis by Spot14
Annual summary 15 Sep 2010-14 Sep 2011
COLORADO UNIV AURORA CO
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The objective of this study is to develop models to causatively link Spot 14 S14 expression in the mammary epithelium and tumor formation, metabolism, and metastasis. Our hypothesis is that S14 promotes de novo lipid biosynthesis in tumor cells, causing enhanced proliferation and ultimately, tumor metastasis. Using a genetic model of spontaneous tumorigenesis, we have found that the presence of S14 significantly accelerates tumor latency, and is associated with branching and budding of the non tumor- bearing mammary glands. Additionally, we have established cell lines from S14-- mammary epithelium, transformed with the Polyoma Middle T antigen PyMT or Neu oncogenes. These lines will be used in xenotransplant experiments to test the requirement of S14 for mammary tumor growth and cellular lipid metabolism.
- Anatomy and Physiology
- Medicine and Medical Research