Personalized Medicine in Veterans with Traumatic Brain Injuries
Annual rept. 1 May 2010-30 Apr 2011
BRONX VETERANS MEDICAL RESEARCH FOUNDATION INC (VETERANS AFFAIRS) NEW YORK
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The study was designed to identify clinically accessible molecular biomarkers of traumatic brain injury TBI prior to definitive clinical diagnosis using high throughput microRNA technology. Specifically, these studies will identify, characterize, and validate microRNA biomarker species whose content in peripheral blood mononuclear cells PBMC could help to distinguish TBI cases within Operation Enduring Freedom OEF and Operation Iraqi Freedom OIF veteran population. During the past year, we conducted a Biomarker Discovery study using a high-throughput Array chip platform and identified 18 candidate TBI small RNA biomarkers. Moreover, using independent Q-PCR assays, we confirmed that thirteen 13 of these candidate small RNA biomarker species are, indeed, significantly down-regulated in PBMC of TBI compared to non-TBI control veteran cases. Additionally, we use an unsupervised clustering analysis and demonstrated that differential regulations of these small RNA biomarkers in clinically accessible blood cells are capable of dissecting TBI and non-TBI OEFOIF veteran cases.
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