Preclinical Studies of Induced Pluripotent Stem Cell-Derived Astrocyte Transplantation in ALS
Annual rept. 30 Sep 2010-29 Sep 2011
JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
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We have made substantial progress in obtaining skin biopsies with subsequent fibroblast cultures of a number of sporadic ALS, familial ALS, and control subjects in the last year. We have collected a variety of ALS phenotypes including slow progressing ALS, primary lateral sclerosis PLS, lower motor neuron only ALS LMN, ALS with frontotemporal dementia ALSFTD and appropriate controls Total of approximately 85 subjects collected to date. In addition, we have also obtained 28 samples from subjects with familial ALS these include SOD1, ANG, FIG4, and FUS mutations. In collaboration with Johns Hopkins Investigators, we have developed 22 FALS iPSC lines, 8 sporadic ALS iPSC lines and 4 control iPSC lines. We have been using a method to allow for long-term differentiation of iPS cells into astroglia.
- Medicine and Medical Research