Laser Capture Microdissection Assisted Identification of Epithelial MicroRNA Expression Signatures for Prognosis of Stage I NSCLC
Annual rept. 7 Sep 2010-6 Sep 2011
HEALTH RESEARCH INC BUFFALO NY
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The primary aim of this project is to laser microdissect stage I lung cancer samples, perform microRNA profiling of the epithelial and stromal components and develop component specific signatures of prognosis. This report summarizes the work performed on the project so far. A significant body of work has been performed to optimize the extraction of RNA from formalin fixed paraffin embedded lung cancer tissue and a working protocol established. These finding are useful to the scientific community and have been submitted for publication. Fifty seven of the proposed 77 stage I lung cancer samples have been microdissected this far into their epithelial and stromal component separated. Enough dissection has been performed to successfully acquire 400 ng of total RNA from each component.
- Anatomy and Physiology
- Medicine and Medical Research