Systemic And Local Vaccination Against Breast Cancer With Minimum Autoimmune Sequelae
Annual rept. 15 sep 2010-14 Sep 2011
WAYNE STATE UNIV DETROIT MI
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Our goal is to eliminate the tumor by vaccination and local ablation to render long-term immune protection without excessive autoimmune sequelae. Complimenting this regimen is systemic modulation of naturalinduced Treg iTreg and intratumoral expression of immune augumenting cytokines. The two aims are to 1 Test the hypothesis that cryosurgery of cytokine enriched tumors amplifies Her-2 vaccine response, and 2 Test the hypothesis that disabling iTreg conversion enhances Her-2 immunity, not autoimmunity. In aim 1, we will measure immune response to tumor associated Her-2 by cryotherapy with and without DNA vaccination and the effect of intratumoral expression of cytokine. In aim 2, we will evaluate the degree of iTreg conversion in tumor bearing mice, measure Her-2 vaccine response in Her-2TIEG1-- mice which do not generate iTreg, and measure the induction of experimental autoimmune thyroiditis in iTreg deficient mice.
- Anatomy and Physiology
- Medicine and Medical Research