The Role Of Rho GTPases In Breast Cancer Migration And Invasion
Annual summary 15 Mar 2010-14 Mar 2011
SLOAN-KETTERING INST FOR CANCER RESEARCH NEW YORK
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Rho-family GTPases are the major regulators of the actin and microtubule cytoskeleton. Regulators of the GTPases include GEFs and GAPs, which control activation and deactivation, respectively, of this family of molecular switches. This study explores the role of the GEF Tiam2 in breast cancer cell proliferation and invasion, which was initially discovered using a siRNA screen in MDA-MB-231 breast cancer cells. Protein expression studies are performed using a panel of cell lines, revealing that Tiam2 expression is upregulated in Ras mutant cells. Additionally, Tiam2 depletion causes MDA-MB-231 cells to form fewer colonies in soft agar colony formation assays. Work continues on this project to determine how Tiam2 contributes to breast cancer proliferation and invasion using both cell culture and mouse models.
- Anatomy and Physiology
- Medicine and Medical Research