Rapid Dispersion of Polymicrobial Wound Biofilms with Depolymerase Enzymes
Annual rept. 4 Oct 2010-3 Oct 2011
MARYLAND UNIV COLLEGE PARK
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During the performance period, we successfully screened 700 bacteriophage genomes bioinformatically for putative depolymerase enzymes, selected approx. 15 initial enzymes for gene synthesis, purified several of these enzymes that displayed proper folding and favorable solubility properties, and began characterizing their anti-biofilm potentials. In parallel, we evaluated dozens of ESKAPE Enterococcus faecium,Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter species organisms for production of biofilm and we extracted and purified biofilm polysaccharide from several of these species.
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