Accession Number:

ADA555009

Title:

Rapid Dispersion of Polymicrobial Wound Biofilms with Depolymerase Enzymes

Descriptive Note:

Annual rept. 4 Oct 2010-3 Oct 2011

Corporate Author:

MARYLAND UNIV COLLEGE PARK

Personal Author(s):

Report Date:

2011-11-01

Pagination or Media Count:

18.0

Abstract:

During the performance period, we successfully screened 700 bacteriophage genomes bioinformatically for putative depolymerase enzymes, selected approx. 15 initial enzymes for gene synthesis, purified several of these enzymes that displayed proper folding and favorable solubility properties, and began characterizing their anti-biofilm potentials. In parallel, we evaluated dozens of ESKAPE Enterococcus faecium,Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter species organisms for production of biofilm and we extracted and purified biofilm polysaccharide from several of these species.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE