Accession Number:

ADA553045

Title:

Cloning and Expressing Recombinant Protective Antigen Domains of B. anthracis

Descriptive Note:

Rept. for Oct 2010-Jul 2011

Corporate Author:

ARMY RESEARCH LAB ADELPHI MD SENSORS AND ELECTRON DEVICES DIRECTORATE

Report Date:

2011-09-01

Pagination or Media Count:

26.0

Abstract:

Protective antigen PA of Bacillus anthracis is a four-domain protein that is required for transport of edema factor EF and lethal factor LF into host cells during anthrax infection. Each of the four domains of PA plays a role in infection host cell receptor binding, domain 4, PA heptamerization and host membrane insertion, domains 2 and 3, and EF and LF recognition and intracellular transport, domain 1. This report details our efforts to produce single- and multi-domain combinations of the PA protein. All multi-domains 1-2, 2-3, 3-4 and three of the four single domains 1, 2, 4 have been cloned, expressed, and purified and will be used for future efforts in domain mapping of PA-binding antibodies and peptide reagents.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE