Detection of the CS20 Colonization Factor Antigen in Diffuse-Adhering Escherichia coli Strains
NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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We analyzed a randomly selected group of 30 diffusely adherent DAEC, 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli ETEC colonization factors CFs were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, th ree DAEC were found to express coli surface antigen 20 CS20. No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host.