Accession Number:

ADA551688

Title:

Feasibility of a DNA-Based Combinatorial Array Recognition Surface (CARS) in a Polyacrylamide Gel Matrix

Descriptive Note:

Journal Article

Corporate Author:

AIR FORCE RESEARCH LAB BROOKS CITY-BASE TX HUMAN EFFECTIVENESS DIRECTORATE/BIOSCIENCES AND PROTECTION DIV

Report Date:

2007-12-12

Pagination or Media Count:

9.0

Abstract:

We report initial attempts al developing a self-assembled combinatorial DNA biosensor array which may be capable of binding and identilYing vir1ually any soluble analyte that binds the array by pattern recognition, in effect making it a universal biosensor surface. Data are presented for differential binding pattcrns of various analytcs to one-dimensional arrays of combinatorial DNA concatamer libraries, which are spatially separatcd according to size and charge by electrophoresis in polyacrylamide gels. These DNA concatamer librarics arc essentially composed of single-stranded ss random DNA 60mers, which fonn a smear pattern in gels following elcctrophoresis. Whcn used to bind and detect various analytcs or mixtures of analytes in the gel, we refer to the DNA smear as a Combinatorial Array Recognition Surface CARS. Differences in intrinsic fluorescence scanning patterns of CARS gel strips were compared before and after addition of various analytes to the arrays to detcct binding pallems. Scans revealed a high level of reproducibility for individual CARS arrays in a given gel with or without bound analytes. Scan patterns between diffcrent CARS gel strips were initially less reproducible, but purification of the DNA library using spin columns prior to electrophoresis improved gel-to-gel reproducibility.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medical Facilities, Equipment and Supplies
  • Elastomers and Rubber

Distribution Statement:

APPROVED FOR PUBLIC RELEASE