Reactivation of Breast Cancer Micrometastases by Senescent Bone Marrow Stroma
Annual rept. 1 Jul 2010-30 Jun 2011
UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY PISCATAWAY
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We developed in vitro injury model of stroma cultured from bone marrow samples from mice and human donors. We confirmed that oxidative and hypoxic damage and estrogen deprivation can induce IL-6 and IL-8 export by human female, premenopausal stroma. We adapted a breast cancer cell co-culture model with injured stroma to determine the effect on stromal capacity to support dormancy. We adapted an osteoclast activation model to determine the capacity of injured stroma to activate osteoclasts. We also adapted a preosteoblast co-culture model to determine the role of osteoblasts in the micrometastatic cell niche responsible for recurrence of the dormant breast cancer. These experiments will serve to identify the adhesion and signaling necessary to maintain survival and resistance of dormant cancer cells.
- Anatomy and Physiology
- Medicine and Medical Research