Accession Number:

ADA550871

Title:

Reactivation of Breast Cancer Micrometastases by Senescent Bone Marrow Stroma

Descriptive Note:

Annual rept. 1 Jul 2010-30 Jun 2011

Corporate Author:

UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY PISCATAWAY

Personal Author(s):

Report Date:

2011-07-01

Pagination or Media Count:

13.0

Abstract:

We developed in vitro injury model of stroma cultured from bone marrow samples from mice and human donors. We confirmed that oxidative and hypoxic damage and estrogen deprivation can induce IL-6 and IL-8 export by human female, premenopausal stroma. We adapted a breast cancer cell co-culture model with injured stroma to determine the effect on stromal capacity to support dormancy. We adapted an osteoclast activation model to determine the capacity of injured stroma to activate osteoclasts. We also adapted a preosteoblast co-culture model to determine the role of osteoblasts in the micrometastatic cell niche responsible for recurrence of the dormant breast cancer. These experiments will serve to identify the adhesion and signaling necessary to maintain survival and resistance of dormant cancer cells.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE