Metabolic Reorganization in Breast Cancer Epithelial Cells: Role of the Pentose Phosphate Shunt
Final rept. 1 May 2008-30 Apr 2011
FLORIDA UNIV GAINESVILLE
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Breast cancer is the most frequently diagnosed cancer in women with about 180,000 new cases reported each year. Early detection allows the greatest chance for successful therapies which include surgical procedures, irradiation, hormonal, and chemical intervention. Yet, these do not always achieve complete recovery, so our goal was to develop novel techniques that might identify markers that would allow us to construct metabolic maps in different types of breast cancers to predict efficacy of therapeutic treatment options. Using NMR, we have now demonstrated that hypoxic treatment of a basal B, triple negative breast cancer cell line increases substantially the flux of non-glycolytic products into the TCA cycle which might increase the ability of cells to use oxidative phosphorylation for ATP production. An inhibitor of the pentose phosphate shunt significantly altered glucose metabolism suggesting that this pathway makes a major contribution to metabolic flux. Identifying the non-glycolytic products that support mitochondrial metabolism will be a major direction in our work, as this impacts our understanding of mitochondrial function in breast cancer.
- Anatomy and Physiology
- Medicine and Medical Research