Development of a Suite of Luciferase Gene Probes for the Screening and Detection of Marine Bioluminescent Systems and Organisms
SOUTHAMPTON UNIV (UNITED KINGDOM)
Pagination or Media Count:
The long-term goal of this project is to integrate molecular information regarding diversity and physiology of bioluminescence into the framework of observational technologies to improve the representation and forecasting of bioluminescent events. The focus of this proposal is a to conduct laboratory and field trials using luciferase gene probes developed in the lab in conjunction with diversity studies to screen for related, yet novel luciferase genes in the genomes of marine bioluminescent organisms. b to detect in situ bioluminescent microorganisms in order to supplement bathyphotometric luminescence measurements with coincident real-time molecular data c to conduct real-time PCR quantification of luciferase mRNA in dinoflagellates under different growth stages d to use bioluminescent molecular markers to search for and isolate novel microscopic bioluminescent organisms.