Distribution and Larval Habitats of Anopheles Species in Northern Gyeonggi Province, Republic of Korea
168TH MULTIFUNCTIONAL MEDICAL BATTALION APO AP 96205-5247 65TH MEDICAL BRIGADE
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A total of 180 larval collection sites e.g., rice paddies, marshes, ground pools, ponds, stream margins and irrigation and drainage ditches was surveyed within a 2 km radius from Warrior Base training area, 5 km south of Panmunjeom Joint Security Area, demilitarized zone, Gyeonggi Province, Republic of Korea ROK, from May through October, 2007 to characterize larval habitat distributions of members of the Anopheles Hyrcanus Group An. sinensis, An. lesteri, An. pullus, An. belenrae, An. kleini, and An. sineroides. A total of 5,859 anopheline larvae was collected from 84.4 of the sites surveyed, of which 4,071 were identified to species by polymerase chain reaction PCR using the ribosomal DNA internal transcribed spacer 2 rDNA ITS2. Anopheles sinensis 52.6 was the most frequently collected, followed by An. kleini 29.4, An. sineroides 9.8, An. pullus 6.7, An. belenrae 1.1, and An. lesteri 0.5. Anopheles pullus and An. kleini were collected in greater proportions in May and from May - July, respectively. Few An. sinensis were collected from May - June, but it was the predominant species collected by August, and accounted for 80 of all larvae from September - October. Anopheles kleini was found in all habitats sampled however, it was collected most frequently in young growth rice paddies, while An. sinensis was collected more frequently in mature and post-harvest paddies. Anopheles pullus was associated with pre-cultivated rice paddies, including water-filled tire ruts left from the previous falls harvest.