Assessment of Regenerative Capacity in the Dolphin
Annual rept. 11 Oct 2010-10 Oct 2011
VET-STEM INC POWAY CA
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Described herein is the technical information pertaining to Year 2 of a multi-year effort to determine and characterize the use of adipose fat-derived stem cells in the treatment of epidermal skin wounds. Adipose tissue was successfully harvested from the nuchal fat pad of six Atlantic Bottlenose dolphins via liposuction cells released during the digestion of the adipose tissue were analyzed for cytology, assayed for the total number of colony-forming cells, expanded in culture, differentiated into multiple cell lineages and analyzed for stem cell surface markers. Cultured cells were also cryogenically frozen for future cell therapy treatment of dolphin skin wounds. Gene array analysis on the cultured cells show that a number of human related stem cell genes are positive. Furthermore, CD markers for known stem cell surface markers have been shown to bind to epitopes present on the cultured cells, lending further proof that the isolated cells are stem cells. Injections of stem cells into the skin wounds of dolphins display a more rapid healing than carrier solution alone.
- Animal Husbandry and Veterinary Medicine