Regulation of Initial Attachment of P. aeruginosa
Final rept. 17 Sep 2007-16 Sep 2010
STATE UNIV OF NEW YORK AT BINGHAMTON RESEARCH FOUNDATION
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The goal of this proposal is to identify biofilm-specific regulatory proteins involved in the regulation of initial biofilm formation that once inactivated, impair or arrest the biofilm formation process. We have identified 8 regulatory proteins which become sequentially phosphorylateddephopshorylated during biofilm formation. Inactivation of sagS and bfiS arrested biofilm formation prior to the maturation-1 stage of biofilm development, as indicated by analyses of biofilm architecture, and protein and phosphoprotein patterns. Discontinuation of sagS and bfiS expression in established biofilms resulted in the collapse of biofilms to an earlier developmental stage indicating a requirement for these regulatory systems for the development and maintenance of normal biofilm architecture. plkR inactivation did not affect biofilm formation while plkR overexpression coincided with dispersion. Downstream gene targets of selected regulators were identified. Inactivation of bfiS coincided with decreased virulence. Inactivation of sagS rendered biofilms as susceptible to antimicrobials as planktonic cells indicating that biofilm resistance is regulated. Findings from this research indicated biofilm formation to be governed by a previously unidentified regulatory program, and provided detailed information how to control or prevent biofilm growth by interrupting the sequence of gene activation and affecting a cells ability to attach to a surface and form deleterious biofilms.
- Genetic Engineering and Molecular Biology