Accession Number:

ADA543825

Title:

The Role of Mitochondrial TCA Cycle Enzymes in Determining Prostate Cancer Chemosensitivity

Descriptive Note:

Annual rept. 1 Mar 2010-28 Feb 2011

Corporate Author:

OREGON HEALTH AND SCIENCE UNIV PORTLAND

Personal Author(s):

Report Date:

2011-03-01

Pagination or Media Count:

7.0

Abstract:

In the needle biopsy samples from high-risk prostate cancer patients, we further confirmed that TCA cycle enzymes MDH2 and CS are elevated in subsets of patients. In vitro, comparing three commonly used prostate cancer cell lines PC3, C42B and LNCaP to benign prostate cell line BPH1 showed that CS expressions are very robust in all cell lines, and MDH2 is overexpressed in the cancer cell lines only. We established stable MDH2 shRNA knockdown cell lines in PC3, C42B and LNCaP. The resulted cells have lower rates of proliferation, lower levels of ATP production, and higher sensitivity to docetaxel chemotherapy compared to the scramble control knockdowns. These confirm our original hypothesis that MDH2 plays a role in determining prostate cancer chemosensitivity.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE