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Novel Serum Proteomic Signatures in a Non-Human Primate Model of Retinal Injury

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To identify candidate protein biomarkers in sera indicative of acute retinal injury. Methods We used laser photocoagulation as a model of acute retinal injury in Rhesus macaques. In a paired-control study design, we collected serum from each animal n6 at 4 h, 1 day, and 3 days following a mock procedure and then again following retinal laser treatment that produced mild lesions. Samples were fractionated by isoelectric focusing, digested with trypsin, and analyzed by liquid chromatographytandem mass spectrometry LC-MSMS. Spectral counting was used to determine relative protein abundances and identify proteins with statistically significant differences between control and treated sera. Results Mild retinal injury was confirmed by fundus photography and histological examination. The average number of total proteins detected by LC-MSMS was 908 82 among samples from all three time points. Following statistical analysis and employing stringent filtering criteria, a total of 19 proteins were identified as being significantly more abundant in sera following laser-induced retinal injury, relative to control sera. Many of the proteins detected were unique to one time point. However, four proteins phosphoglycerate kinase 1, keratin 18, Lewis alpha-3-fucosyltransferase, and ephrin receptor A2 showed differences that were significant at both 4 h and 1 day after laser treatment, followed by a decrease to baseline levels by day 3. Conclusions A serum biomarker response to mild retinal laser injury was demonstrated in a primate model. Among the proteins detected with highest significant differences, most are upregulated within 24 h, and their appearance in the serum is transient. It is conceivable that a panel of these proteins could provide a means for detecting the acute-phase response to retinal injury. Further investigation of these candidate biomarkers and their correlation to retinal damage is warranted.

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  • Medicine and Medical Research

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