A Novel Combination of Thermal Ablation and Heat-Inducible Gene therapy for Breast Cancer Treatment
Annual summary rept. 31 Mar 2006-31 Mar 2009
DUKE UNIV DURHAM NC DEPT OF MECHANICAL ENGINEERING AND MATERIALS SCIENCE
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High intensity focused ultrasound HIFU has been developed as an emerging non-invasive strategy for cancer treatment by thermal ablation of tumor tissue. The feasibility of synergistic combination of HIFU thermal ablation and HIFU-induced gene therapy is interpretedboth in vitro and in vivo using cancer cell lines HeLa, 4T1 and R3230Ac transfected with mark gene encoding proteins GFP and Luciferaseunder the control of Hsp70B promoter. Moreover, a transparent cell-embedded hydrogel is developed to allow for simultaneous assessment of lesion formation, gene expression and temperature distribution. This work opens up a new paradigm for synergistic combination of HIFU thermal ablation with heat-induced gene therapy to improve the overall quality and efficacy of breast cancer therapy.
- Medicine and Medical Research
- Stress Physiology