Accession Number:

ADA539740

Title:

Experimental Therapeutics Against the Toxic and Lethal Effects Resulting from Acute Exposure to Nerve Agents Without Carbamate Pretreatment in Guinea Pigs

Descriptive Note:

Technical rept. 1 Mar 2009-30 Jun 2010

Corporate Author:

ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Report Date:

2010-09-01

Pagination or Media Count:

38.0

Abstract:

Our current countermeasure against nerve agent poisoning involves carbamate pretreatment followed by a post-exposure therapy atropine and oxime. For militarycivilian populations not enrolled in the carbamate pretreatment program, atropine and oxime may not offer adequate protection against the toxiclethal effects of nerve agents. To reduce the reliance on carbamate pretreatment, we have developed a post-exposure therapy mix that can protect against the lethal effect of a 2xLD50 dose of tabun, sarin, soman, cyclo-sarin or VX. Guinea pigs chronically instrumented for concurrent recordings of EEG, cardiorespiratory activities, diaphragm and skeletal muscle EMG were used in this study. The post-exposure therapy mix consisted of scopolamine 0.5 mgkg, methylatropine 2 mgkg, physostigmine 0.015 mgkg, MMB4 26.1 mgkg, and phenobarbital 25 mgkg. Results showed that only mild and short-lasting acute cholinergic effects salivation, dystonia, tremor were seen after exposuretherapy. During convalescence, none of the animals exhibited seizuresconvulsions, signs of anomalous cardiorespiratory activities, or any debilitating effects. The animals were asymptomatic within 30 min following therapy and survived the agent challenge 24 hr later. In conclusion, the therapy mix used in this study was effective not only in antagonizing nerve agent-induced lethality, but also in protecting the functional integrity of the CNS and cardiorespiratory system.

Subject Categories:

  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE