Connective Tissue Growth Factor (CTGF) as a Regulator of Lactogenic Differentiation
UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD F EDWARD HEBERT SCHOOL OF MEDICINE
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The degree of differentiation of mammary epithelial cells is dependent on their response to basement membrane and stromal protein-induced signals. Connective Tissue Growth Factor CTGFCCN2, a known stromal mediator, is highly upregulated by dexamethasone and is required for the lactogenic differentiation of mouse mammary epithelial cells. Using a Tet-off system to overexpress CTGFCCN2 in the HC11 cell background, we found that its elevated expression enhanced multiple markers of lactogenic differentiation, including beta-casein transcription, mammosphere formation, and stabilization of Stat5 activity. Elevated levels of CTGFCCN2 partially abrogated the requirement of matrix proteins for the transcription of beta-casein, suggesting that CTGFCCN2 contributes to lactogenic differentiation through a regulation of cellmatrix adhesion.
- Anatomy and Physiology